ABSTRACT
Objective: To simultaneously determinate the content of chlorogenic acid, amygdalin, gardenoside, hesperidin, baicalin, wogonoside, ammonium glycyrrhizate and schisandrin in Qingfei Decoction by HPLC-VWD, and establish the fingerprint of Qingfei Decoction. The results can be applied to characterize and distinguish commercially available Qingfei Decoction preparations from different manufacturers. Methods: Agilent Technologies Zorbax SB-C18 (250 mm × 4.6 mm, 3.5 μm) column was used, with acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as the mobile phase for gradient elution, column temperature of 35 ℃, flow rate of 0.6 mL/min, injection volume of 10 μL. The content of the indicator components and the fingerprints were simultaneously assayed by the above method. Fingerprint similarity evaluation, heat map cluster analysis and other methods were used to characterize and distinguish the commercially available Qingfei Decoction preparations from different sources. Results: The specificity, linear relationship (r2 > 0.999 8), precision (RSD ≤ 1.85%, n = 6), repeatability (RSD ≤ 1.82%, n = 6), stability (RSD ≤ 1.49%, 48 h ) of eight components were good; The recovery rate of the sample was in the range of (93.19 ± 1.93)% to (102.36 ± 4.17)% (n = 6). The results of content determination showed that the daily dosage of the indicator ingredients in the marketed Qingfei Decoction preparations fluctuated greatly in different manufacturers, such as baicalin, which fluctuated from 59.85 to 224.05 mg, but the fluctuation was not obvious in different dosage forms of the same manufacturer, for example, the content of baicalin in the two preparations of KPC Herbs was 140.00 to 142.47 mg. At the same time, the similarity calculation results of fingerprints were all greater than 0.945, which indicated that the fingerprints of Qingfei Decoction from different sources were highly similar. Conclusion: The method is accurate and reliable with good repeatability. It can be used to determine the content of eight components in the commercial Qingfei Decoction preparations and establish the fingerprint of Qingfei Decoction. The commercial Qingfei Decoction preparations can be characterized by heat map clustering analysis of the daily dosage of the eight components and the fingerprint similarity evaluation to distinguish the commercially available preparations of Qingfei Decoction from different sources.
ABSTRACT
Objective: To optimize the optional extraction process parameters of Modified Simiao Granule (MSG). Methods: HPLC was employed to determine the content of rhodoglycoside, chlorogenic acid, ammonium glycyrrhizate, berberine hydrochloride, and glycyrrhizin, using the extraction ratio, dried cream yield, and fingerprint similarity of which as comprehensive evaluation indexes, orthogonal test was used to investigate effects of solid-liquid ratio, extraction time and times on extraction process. Information entropy method was used to determine the objective weight of each index to achieve orthogonal test optimization of extraction process. Results: According to the result of comprehensive score, the optimal extraction conditions of this preparation were as follows: extracted twice with 10 times of water quantity, each for 30 min. The mean of the three batches of comprehensive evaluation was 88.91 and the RSD value was 0.61%. Conclusion: The optimized technologies are stable, repeatable, feasible and suitable for the industrial production of MSG.
ABSTRACT
AIM To develop an HPLC method for the simultaneous content determination of five constituents in Chairen Capsules (Bupleuri Radix,Ziziphi spinosae Semen,Chuanxiong Rhizoma,etc.).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic Merck Purospher STAR C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,290 and 335 nm.RESULTS Spinosin,ferulic acid,cinnamaldehyde,liquiritin and ammonium glycyrrhizate showed good linear relationships within the ranges of 0.009 26-0.092 6 μg (R2 =0.999 6),0.056 2-0.562 μg (R2 =0.999 8),0.130-1.30 μg (R2 =0.999 8),0.275-2.75 μg (R2 =0.999 5) and 0.568-5.68 μg (R2 =0.999 2),whose average recoveries were 100.73％,100.62％,100.02％,100.41％ and 99.96％ ％ with the RSDs of 2.20％,1.37％,1.52％,1.30％ and 0.80％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Chairen Capsules.